Amsbio reports how the Sasaki Lab within the School of Veterinary Medicine at the University of Pennsylvania (USA) devised a protocol in which induced pluripotent stem cells (iPSCs) could be utilized to produce reconstituted human testes.
Since the original 2020 publication of their groundbreaking research, Dr Kotaro Sasaki’s team has made significant progress in reconstituting human testis-like tissue from iPSCs.
A 2025 blog update describes a new protocol for generating xenogeneic reconstituted testes (xrTestes) that support human spermatogonial development in vitro. These findings strengthen the potential of the Sasaki Lab system as a valuable model for studying early human spermatogenesis and offer a practical and scalable platform for further research in reproductive biology. The strides made by researchers in the Sasaki Lab are paving a new way for studies to explore treatments for male infertility.
To support reproductive biology research, Amsbio offers a wide range of reagents for stem cell culture, differentiation, and 3D tissue modelling.
The Sasaki Lab chose to use StemFit® feeder-free, chemically defined stem cell culture media from Amsbio to maintain their iPSC culture. In addition, the xrTestes cells were cultured on plates coated in recombinant laminin E8 fragments using iMatrix-511 silk from Amsbio. The Sasaki lab also cryopreserved their cells using CELLBANKER 1 from Amsbio, a trusted solution for the storage of any cell type including sensitive cell lines. CELLOTION™ cell wash and recovery solution from Amsbio was also used by the lab to collect FACS-sorted in vitro cells prior to single-cell RNA-seq library preparation.
To learn more about the Sasaki Labs most advances in developing reconstituted human testes cells from iPSCs read the full 2025 blog update at https://www.amsbio.com/news/reconstituting-human-testes-from-ipscs
For further information on Stem Cell Synergy Solutions from Amsbio please visit https://www.amsbio.com/research-areas/stem-cells or contact the company on +31-72-8080244 / +44-1235-828200 / +1-617-945-5033 / info@amsbio.com.
